Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1) (N-Term) antibody

Antigen: Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1)

Synonyms: P62DOK, MGC117395, MGC138860

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392070

Additional Information

Alternative name: DOK1

Gene ID: 1796

UniProt: Q99704

Immunogen: This DOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17~46 amino acids from the N-terminal region of human DOK1.

Isotype: Ig

Description: Other names: p62(dok), Downstream of tyrosine kinase 1, pp62

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This DOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17~46 amino acids from the N-terminal region of human DOK1.

Molecular Weight: 52391 DA

Comments:

Background: DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. DOK2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Cell Structure, Cancer

Restrictions: For Research Use only

Images

Western blot analysis of anti-DOK1 Pab (ABIN392070) in HL-60 cell lysate. DOK1 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of DOK1 (arrow) using DOK1 Antibody (N-term) (ABIN392070). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DOK1 gene (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Nelms, Snow, Noben-Trauth: "Dok1 encoding p62(dok) maps to mouse chromosome 6 and human chromosome 2 in a region of translocation in chronic lymphocytic leukemia." in: Genomics, Vol. 53, Issue 2, pp. 243-5, 1998 (PubMed).

Némorin, Duplay: "Evidence that Llck-mediated phosphorylation of p56dok and p62dok may play a role in CD2 signaling." in: The Journal of biological chemistry, Vol. 275, Issue 19, pp. 14590-7, 2000 (PubMed).

Hubert, Ferreira, Debre et al.: "Molecular cloning of a truncated p62Dok1 isoform, p22Dok(del)." in: European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics, Vol. 27, Issue 3, pp. 145-8, 2000 (PubMed).

Liang, Wisniewski, Strife et al.: "Phosphatidylinositol 3-kinase and Src family kinases are required for phosphorylation and membrane recruitment of Dok-1 in c-Kit signaling." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13732-8, 2002 (PubMed).

Yamakawa, Tsuchida, Sugino: "The rasGAP-binding protein, Dok-1, mediates activin signaling via serine/threonine kinase receptors." in: The EMBO journal, Vol. 21, Issue 7, pp. 1684-94, 2002 (PubMed).