|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1) (N-Term) antibody
|Synonyms||P62DOK, MGC117395, MGC138860|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), ELISA
|5 references available|
|Quantity||0.1 mg (0.25 mg/ml)|
|Price||280.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Immunogen||This DOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17~46 amino acids from the N-terminal region of human DOK1.|
|Description||Other names: p62(dok), Downstream of tyrosine kinase 1, pp62|
|Characteristics||Purified Rabbit Polyclonal Antibody (Pab)|
|Specificity||This DOK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17~46 amino acids from the N-terminal region of human DOK1.|
|Molecular Weight||52391 DA|
Background: DOK1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. DOK1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. DOK2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.
|Application Notes||The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Flow cytometry|
|Buffer||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles|
|Research Area||Stem Cells, Cell Structure, Cancer|
|Restrictions||For Research Use only|
Nelms, Snow, Noben-Trauth: "Dok1 encoding p62(dok) maps to mouse chromosome 6 and human chromosome 2 in a region of translocation in chronic lymphocytic leukemia." in: Genomics, Vol. 53, Issue 2, pp. 243-5, 1998 (PubMed).
Némorin, Duplay: "Evidence that Llck-mediated phosphorylation of p56dok and p62dok may play a role in CD2 signaling." in: The Journal of biological chemistry, Vol. 275, Issue 19, pp. 14590-7, 2000 (PubMed).
Hubert, Ferreira, Debre et al.: "Molecular cloning of a truncated p62Dok1 isoform, p22Dok(del)." in: European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics, Vol. 27, Issue 3, pp. 145-8, 2000 (PubMed).
Liang, Wisniewski, Strife et al.: "Phosphatidylinositol 3-kinase and Src family kinases are required for phosphorylation and membrane recruitment of Dok-1 in c-Kit signaling." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13732-8, 2002 (PubMed).
Yamakawa, Tsuchida, Sugino: "The rasGAP-binding protein, Dok-1, mediates activin signaling via serine/threonine kinase receptors." in: The EMBO journal, Vol. 21, Issue 7, pp. 1684-94, 2002 (PubMed).