DOK1 antibody (pTyr398)

Catalog No. ABIN6256732

The Rabbit Polyclonal anti-DOK1 antibody (ABIN6256732) specifically detects DOK1 in WB, ELISA, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DOK1 antibody (pTyr398) (ABIN6256732)

Target: DOK1 (Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-DOK1 Antibody

Binding Specificity: pTyr398

Specificity: Phospho-p62 Dok (Tyr398) Antibody detects endogenous levels of p62 Dok only when phosphorylated at Tyrosine 398.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human p62 Dok around the phosphorylation site of Tyr398.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DOK1

Target: DOK1 (Docking Protein 1, 62kDa (Downstream of tyrosine Kinase 1) (DOK1))

Alternative Name: DOK1

Background:

Description: DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.

Gene: DOK1

Molecular Weight: 62kDa

Gene ID: 1796

UniProt: Q99704