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DOK2 (AA 380-412), (C-Term) antibody

Details for Product No. ABIN392072, Supplier: Log in to see
Antigen
  • DokR
  • Frip
  • Dok-2
  • p56DOK
  • p56dok-2
Epitope
AA 380-412, C-Term
41
26
13
13
13
5
3
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
160
64
34
2
2
1
Host
Rabbit
146
14
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
6
6
6
4
4
4
4
4
4
4
4
4
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
105
59
44
36
34
23
8
3
1
1
1
Supplier
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Immunogen This DOK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 380-412 AA from the C-terminal region of human DOK2.
Clone RB1757
Isotype Ig
Specificity This DOK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383~412 amino acids from the C-terminal region of human DOK2.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background Docking proteins interact with receptor tyrosine kinases and mediate particular biological responses using signal transduction. Dok-2 acts as a multiple docking protein downstream of receptor or non-receptor tyrosine kinases. By this mechanism it acts to negatively regulate signal transduction and cell proliferation controlled by cytokines in a feedback loop. Dok-2 is highly expressed in cells and tissues of hematopoietic origin as well as in lung. Expression of bcr/abl induces additional tyrosine phosphorylation of the Dok1 and Dok2 proteins and their association with Ras-GAP. Thus, it is suspected that DOK association regulates GAP activity toward Ras and that the Dok proteins serve as mediators of bcr-abl signaling. The role of Dok proteins in bcr-abl regulation may also be implicated in chronic myelogenous leukemia (CML), which is characterized by a Philadelphia chromosome translocation t(9,22). Such a mutation would result in a p210-bcr/abl chimeric protein-tyrosine kinase which has been found in many CML cases.
Synonyms: p56(dok-2), Docking protein 2, Downstream of tyrosine kinase 2
Molecular Weight 45548 DA
Gene ID 9046
UniProt O60496
Research Area Stem Cells, Cell Signaling, Cancer, Cell Cycle, Signaling, Chromatin, Cell Structure
Pathways
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-DOK2 (AA 380-412), (C-Term) antibody (ABIN392072) Western blot analysis of anti-DOK2 Pab (ABIN392072) in 174xCEM cell lysate. DOK2 (Arr...
Western Blotting (WB) image for anti-DOK2 (AA 380-412), (C-Term) antibody (ABIN392072) Western blot analysis of DOK2 (arrow) using DOK2 Antibody (C-term) (ABIN392072). 293 ...
Immunohistochemistry (IHC) image for anti-DOK2 (AA 380-412), (C-Term) antibody (ABIN392072) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Salomon, Ficarro, Brill et al.: "Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 443-8, 2003 (PubMed).

Di Cristofano, Carpino, Dunant et al.: "Molecular cloning and characterization of p56dok-2 defines a new family of RasGAP-binding proteins." in: The Journal of biological chemistry, Vol. 273, Issue 9, pp. 4827-30, 1998 (PubMed).