B Lymphoid Tyrosine Kinase (BLK) (N-Term) antibody

Antigen: B Lymphoid Tyrosine Kinase (BLK)

Synonyms: MGC10442, MGC112647, BLK, MGC142356, MGC136231, zgc:136231

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392087

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: BLK (N-term)

Gene ID: 640

Swiss-Prot: P51451

Immunogen: This BLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human BLK.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: B lymphoid tyrosine kinase

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human BLK.

Molecular Weight: 57607 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism

Restrictions: For Research Use only

Images

Western blot analysis of anti-BLK Pab (ABIN392087) in mouse kidney tissue lysate. BLK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of anti-BLK Pab (ABIN392087) transiently transfected HEK-293 cell line lysate (1ug/lane). BLK pab(arrow) was detected using the purified Pab (1:1500 dilution).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.