Neurotrophin 3 (NTF3) (Center) antibody

Antigen: Neurotrophin 3 (NTF3)

Synonyms: NT3, HDNF, NGF2, NGF-2, MGC129711, NT-3, ntf3-pen, wu:fl98d04, nt3, hdnf, ngf2, nt-3, ngf-2, MGC84157, NTF3

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392182

Additional Information

Alternative name: NTF3

Gene ID: 4908

UniProt: P20783

Immunogen: This NTF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130~159 amino acids from the center region of human NTF3.

Isotype: Ig

Description: Other names: Neurotrophin-3 [Precursor], NT-3, Neurotrophic factor, HDNF, Nerve growth factor 2, NGF-2, NTF3

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NTF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130~159 amino acids from the center region of human NTF3.

Molecular Weight: 29355 DA

Comments:

Background: NTF3 is a member of the neurotrophin family that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting ~~ 1:50~100 Immunohistochemistry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Stem Cells, Neurology

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human brain tissue reacted with NTF3 antibody (Center) (ABIN392182), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

NTF3 Antibody (Center) (ABIN392182) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NTF3 antibody detected the NTF3 protein (arrow).

Publications

Product: Kalcheim, Carmeli, Rosenthal: "Neurotrophin 3 is a mitogen for cultured neural crest cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 5, pp. 1661-5, 1992 (PubMed).