Receptor-Interacting Serine-threonine Kinase 2 (RIPK2) (C-Term) antibody

Antigen: Receptor-Interacting Serine-threonine Kinase 2 (RIPK2)

Synonyms: CCK, RICK, RIP2, CARD3, GIG30, CARDIAK, RIPK2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392274

Additional Information

Alternative name: RIPK2

Gene ID: 8767

UniProt: NP_003812

Immunogen: This RIPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 467~496 amino acids from the C-terminal region of human RIPK2.

Isotype: Ig

Description: Other names: Receptor-interacting serine/threonine protein kinase 2, RIP-like interacting CLARP kinase, Receptor-interacting protein 2, RIP-2, CARD-containing interleukin-1 beta converting enzyme associated kinase, CARD-containing IL-1 beta ICE-kinase, RIP2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This RIPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 467~496 amino acids from the C-terminal region of human RIPK2.

Molecular Weight: 61194 DA

Comments:

Background: RIPK2 is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases. The encoded protein contains a C-terminal recruitment domain (CARD), and is a component of signaling complexes in both the innate and adaptive immune pathways. It is a potent activator of NF-kappaB and inducer of apoptosis in response to various stimuli.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.Preca

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-RIPK2 Pab (ABIN392274) in Ramos cell line lysates (35ug/lane). RIPK2 (arrow) was detected using the purified Pab.

Western blot analysis of anti-RIPK2 Pab (ABIN392274) in mouse cerebellum tissue lysate. RIPK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Chin, Dempsey, Bruhn et al.: "Involvement of receptor-interacting protein 2 in innate and adaptive immune responses." in: Nature, Vol. 416, Issue 6877, pp. 190-4, 2002 (PubMed).

Munz, Hildt, Springer et al.: "RIP2, a checkpoint in myogenic differentiation." in: Molecular and cellular biology, Vol. 22, Issue 16, pp. 5879-86, 2002 (PubMed).

Chen, Clark: "Equilibrium and kinetic folding of an alpha-helical Greek key protein domain: caspase recruitment domain (CARD) of RICK." in: Biochemistry, Vol. 42, Issue 20, pp. 6310-20, 2003 (PubMed).