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Details for Product No. ABIN392555

PI3KC3 (N-Term), (AA 24-53) antibody

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Antigen
Epitope
»Alternatives N-Term, AA 24-53
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality (Clone) Polyclonal ()
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Pubmed 2 references available
Catalog no. ABIN392555
Quantity 400 µL
Price
291.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 AA from the N-terminal region of human PI3KC3.
Clone RB1695
Isotype Ig
Specificity This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32~61 amino acids from the N-terminal region of human autophagy kinase PI3KC3.
Predicted Reactivity Mouse (Murine),Pig (Porcine),Rat (Rattus),Xenopus laevis
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Background PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.
Synonyms: Phosphatidylinositol 3-kinase catalytic subunit type 3, PtdIns-3-kinase type 3, PI3-kinase type 3, PI3K type 3, Phosphoinositide-3-kinase class 3, Phosphatidylinositol 3-kinase p100 subunit, vps34, PI 3 Kinase Class 3
Molecular Weight 101549 DA
Gene ID 5289
UniProt Q8NEB9
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Autophagy
Application Notes Recommend dilutions: WB : 1:1000,IHC : 1:50-100,IF : 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 1.64 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Background publications Volinia, Dhand, Vanhaesebroeck et al.: "A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system." in: The EMBO journal, Vol. 14, Issue 14, pp. 3339-48, 1995 (PubMed).

Vergne, Chua, Deretic: "Tuberculosis toxin blocking phagosome maturation inhibits a novel Ca2+/calmodulin-PI3K hVPS34 cascade." in: The Journal of experimental medicine, Vol. 198, Issue 4, pp. 653-9, 2003 (PubMed).

Alternatives for antigen "PI3KC3", type "Antibodies"
Hosts (14)
Reactivities (14), (1), (1), (1), (1), (1)
Applications (8), (7), (6), (2), (1)
Epitopes (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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