NUAK1 antibody (C-Term)

Catalog No. ABIN392640

This anti-NUAK1 antibody is a Rabbit Polyclonal antibody detecting NUAK1 in WB and IHC (p). Suitable for Human and Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for NUAK1 antibody (C-Term) (ABIN392640)

Target: NUAK1 (NUAK Family, SNF1-Like Kinase, 1 (NUAK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB3521

Product Details anti-NUAK1 Antibody

Binding Specificity: AA 604-634, C-Term

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This ARK5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 604-634 amino acids from the C-terminal region of human ARK5.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-NUAK1 antibody (ABIN392640)

Humbert, Navaratnam, Augert, Da Costa, Martien, Wang, Martinez, Abbadie, Carling, de Launoit, Gil, Bernard: "Regulation of ploidy and senescence by the AMPK-related kinase NUAK1." in: The EMBO journal, Vol. 29, Issue 2, pp. 376-86, (2010) (PubMed).

Target Details for NUAK1

Target: NUAK1 (NUAK Family, SNF1-Like Kinase, 1 (NUAK1))

Alternative Name: ARK5

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Molecular Weight: 74305

Gene ID: 9891

NCBI Accession: NP_055655

UniProt: O60285