NUAK1 antibody (AA 362-661)

Catalog No. ABIN7268966

The Rabbit Polyclonal anti-NUAK1 antibody is suitable to detect NUAK1 in samples from Human. It has been validated for WB.

Quick Overview for NUAK1 antibody (AA 362-661) (ABIN7268966)

Target: NUAK1 (NUAK Family, SNF1-Like Kinase, 1 (NUAK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUAK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NUAK1 Antibody

Binding Specificity: AA 362-661

Purpose: NUAK1 Rabbit pAb

Sequence: LERQRSLKKS KKENDFAQSG QDAVPESPSK LSSKRPKGIL KKRSNSEHRS HSTGFIEGVV GPALPSTFKM EQDLCRTGVL LPSSPEAEVP GKLSPKQSAT MPKKGILKKT QQRESGYYSS PERSESSELL DSNDVMGSSI PSPSPPDPAR VTSHSLSCRR KGILKHSSKY SAGTMDPALV SPEMPTLESL SEPGVPAEGL SRSYSRPSSV ISDDSVLSSD SFDLLDLQEN RPARQRIRSC VSAENFLQIQ DFEGLQNRPR PQYLKRYRNR LADSSFSLLT DMDDVTQVYK QALEICSKLN

Cross-Reactivity: Human, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 362-661 of human NUAK1 (NP_055655.1).

Isotype: IgG

Application Details

Application Notes: WB,1:200 - 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for NUAK1

Target: NUAK1 (NUAK Family, SNF1-Like Kinase, 1 (NUAK1))

Alternative Name: NUAK1

Background: Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1 complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate in transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair.,NUAK1,ARK5,Cancer,Signal Transduction,Kinase,Serine/threonine kinases,NUAK1

Molecular Weight: 23kDa/74kDa

Gene ID: 9891

UniProt: O60285