NIMA (Never in Mitosis Gene A)-Related Kinase 1 (NEK1) (C-Term) antibody

Antigen: NIMA (Never in Mitosis Gene A)-Related Kinase 1 (NEK1)

Synonyms: KIAA1901, MGC138800, NY-REN-55, DKFZp686D06121, DKFZp686K12169, kat, MGC189817, D8Ertd790e

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Hamster

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392648

Additional Information

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Alternative name: NEK1 (C-term)

Gene ID: 4750

Swiss-Prot: Q96PY6

Immunogen: This NEK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1173~1202 amino acids from the C-terminal region of human NEK1.

Isotype: Ig  (Matching secondary antibodies)

Description: Other names: Serine/threonine-protein kinase Nek1, NimA-related protein kinase 1, NY-REN-55 antigen

Specificity: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human NEK1.

Molecular Weight: 142828 DA

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism

Restrictions: For Research Use only

Images

The anti-NEK1 Pab (ABIN392648) is used in Western blot to detect NEK1 in CHO cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.