NIMA-Related Kinase 1 (NEK1) (C-Term) antibody

Antigen: NIMA-Related Kinase 1 (NEK1)

Synonyms: KIAA1901, MGC138800, NY-REN-55, DKFZp686D06121, DKFZp686K12169

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Hamster

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392648

Additional Information

Alternative name: NEK1

Gene ID: 4750

UniProt: Q96PY6

Immunogen: This NEK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1173~1202 amino acids from the C-terminal region of human NEK1.

Isotype: Ig

Description: Other names: Serine/threonine-protein kinase Nek1, NimA-related protein kinase 1, NY-REN-55 antigen

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This NEK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1173~1202 amino acids from the C-terminal region of human NEK1.

Molecular Weight: 142828 DA

Comments:

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cell Cycle, Transcription Factors, Signaling, Metabolism

Restrictions: For Research Use only

Images

The anti-NEK1 Pab (ABIN392648) is used in Western blot to detect NEK1 in CHO cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Letwin, Mizzen, Motro et al.: "A mammalian dual specificity protein kinase, Nek1, is related to the NIMA cell cycle regulator and highly expressed in meiotic germ cells." in: The EMBO journal, Vol. 11, Issue 10, pp. 3521-31, 1992 (PubMed).

Surpili, Delben, Kobarg: "Identification of proteins that interact with the central coiled-coil region of the human protein kinase NEK1." in: Biochemistry, Vol. 42, Issue 51, pp. 15369-76, 2003 (PubMed).