Protein Kinase C and Casein Kinase Substrate in Neurons 2 (PACSIN2) (C-Term) antibody

Antigen: Protein Kinase C and Casein Kinase Substrate in Neurons 2 (PACSIN2)

Synonyms: SDPII, AI197433, SdpII, MGC77519, zgc:77519, sdpii, pacsin1, x-PACSIN2, PACSIN2, MGC137898, DKFZp459J1025

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392666

Additional Information

Alternative name: PACSIN2

Gene ID: 11252

UniProt: Q9UNF0

Immunogen: This PACSIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349~379 amino acids from the C-terminal region of human PACSIN2.

Isotype: Ig

Description: Other names: Protein kinase C and casein kinase substrate in neurons protein 2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This PACSIN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 349~379 amino acids from the C-terminal region of human PACSIN2.

Molecular Weight: 55738 DA

Comments:

Background: PACSIN may play a role in vesicle formation and transport. This protein homo- and hetero-aggregates with other PACSINs. It also binds dynamin 1, synaptojanin, synapsin 1 and the neural Wiskott-Aldrich syndrome protein (N-WASP). The protein exhibits a cvesicle-like cytoplasmic distribution and is ubiquitously expressed. PACSIN is phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). The protein contains 1 FCH domain and 1 SH3 domain.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100 Immunofluorescence~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

The anti-PACSIN2 Pab (ABIN392666) is used in Western blot to detect PACSIN2 in T-47D cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Dunham, Shimizu, Roe et al.: "The DNA sequence of human chromosome 22." in: Nature, Vol. 402, Issue 6761, pp. 489-95, 1999 (PubMed).

Strausberg, Feingold, Grouse et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, 2002 (PubMed).