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Diacylglycerol Kinase, delta 130kDa (DGKD) (AA 1061-1091), (C-Term) antibody

Details for Product No. ABIN392725, Supplier: Log in to see
Antigen
  • AI841987
  • D330025K09
  • dgkd-2
  • DGKdelta
  • RGD1563309
Alternatives
anti-Human Diacylglycerol Kinase, delta 130kDa antibody for Immunofluorescence
Epitope
AA 1061-1091, C-Term
20
2
1
1
1
1
1
Reactivity
Human
33
3
Host
Rabbit
34
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
31
21
17
4
4
1
1
Supplier
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Immunogen This DGKD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061-1091 AA from the C-terminal region of human DGKD.
Clone RB3865
Isotype Ig
Specificity This DGKD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1050~1079 amino acids from the C-terminal region of human DGKD.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name DGKD (DGKD Antibody Abstract)
Background Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA &
Synonyms: Diacylglycerol kinase delta, Diglyceride kinase, DAG kinase delta, 130 kDa diacylglycerol kinase
Molecular Weight 132825 DA
Gene ID 8527
UniProt Q16760
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Transcription Factors, Signaling, Metabolism, Cell Structure
Pathways EGFR Signaling Pathway
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Diacylglycerol Kinase, delta 130kDa (DGKD) (AA 1061-1091), (C-Term) antibody (ABIN392725) Western blot analysis of anti-anti-DGK delta Pab (ABIN392725) in Jurkat cell line lys...
Immunohistochemistry (IHC) image for anti-Diacylglycerol Kinase, delta 130kDa (DGKD) (AA 1061-1091), (C-Term) antibody (ABIN392725) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Background publications Sakane, Imai, Kai, Wada, Kanoh: "Molecular cloning of a novel diacylglycerol kinase isozyme with a pleckstrin homology domain and a C-terminal tail similar to those of the EPH family of protein-tyrosine kinases." in: The Journal of biological chemistry, Vol. 271, Issue 14, pp. 8394-401, 1996 (PubMed).