Membrane Associated Guanylate Kinase, WW and PDZ Domain Containing 1 (MAGI1) (C-Term) antibody

Antigen: Membrane Associated Guanylate Kinase, WW and PDZ Domain Containing 1 (MAGI1)

Synonyms: AIP3, BAP1, WWP3, BAIAP1, MAGI-1, TNRC19, CNKSR3, MAGI1

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN392808

Additional Information

Alternative name: BAIAP1 (MAGI1)

Gene ID: 9223

UniProt: NP_004733

Immunogen: This BAIAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1234~1264 amino acids from the C-terminal region of human BAIAP1.

Isotype: Ig

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This BAIAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1234~1264 amino acids from the C-terminal region of human BAIAP1.

Molecular Weight: 136981 DA

Comments:

Background: BAIAP1 is a member of the membrane-associated guanylate kinase homologue (MAGUK) family. Characterized by two WW domains, a guanylate kinase domain, and five PDZ domains, this protein interacts with the cytoplasmic region of BAI1. Together, these proteins may play a role in cell adhesion and signal transduction.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1,000 Western blotting~~1:100~500 Immunohistochemistry~~1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Cell Signaling, Cell Structure

Restrictions: For Research Use only

Images

The anti-BAIAP1 Pab (ABIN392808) is used in Western blot to detect BAIAP1 in mouse brain tissue lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated. BC = breast carcinoma. HC = hepatocarcinoma.

Publications

Product: Pirozzi, McConnell, Uveges et al.: "Identification of novel human WW domain-containing proteins by cloning of ligand targets." in: The Journal of biological chemistry, Vol. 272, Issue 23, pp. 14611-6, 1997 (PubMed).

Wood, Yuan, Margolis et al.: "Atrophin-1, the DRPLA gene product, interacts with two families of WW domain-containing proteins." in: Molecular and cellular neurosciences, Vol. 11, Issue 3, pp. 149-60, 1998 (PubMed).

Shiratsuchi, Futamura, Oda et al.: "Cloning and characterization of BAI-associated protein 1: a PDZ domain-containing protein that interacts with BAI1." in: Biochemical and biophysical research communications, Vol. 247, Issue 3, pp. 597-604, 1998 (PubMed).

Patrie, Drescher, Welihinda et al.: "Interaction of two actin-binding proteins, synaptopodin and alpha-actinin-4, with the tight junction protein MAGI-1." in: The Journal of biological chemistry, Vol. 277, Issue 33, pp. 30183-90, 2002 (PubMed).