APEX1 antibody

Catalog No. ABIN396973

This Rabbit Polyclonal antibody specifically detects APEX1 in WB, IHC, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for APEX1 antibody (ABIN396973)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-APEX1 Antibody

Specificity: Specific to the human APE/ref-1protein. Competitively inhibited from recognizing the APE/ref-1 antigen in tissues using APE/ref-1 protein. Inhibits repair activity of ape protein.

Purification: Immunoaffinity purified

Immunogen: Affinity purified human Apurinic/Apyrimidinic Endonuclease (APE/ref-1) fusion protein.

Type of Immunogen: Fusion protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P (1:250), WB (1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:250. Western Blot: 1:500 (ECL). Detects a single band at 37kD. Diluted antibody can be reused 1-3 times in Western Blot analysis. Apoptosis: Can be used following the apoptosis (TUNEL) procedure using the Boehringer-Mannheim TUNEL assay kit. Antibody staining should be performed AFTER the TUNEL assay. Positive control: HeLa whole cell extract.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.05 % sodium azide before the addition of glycerol to 40 %.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APEX1 / APE1

Background: Name/Gene ID: APEX1

Synonyms: APEX1, AP endonuclease class I, AP lyase, APE, APE-1, APEN, APE1, APEX nuclease, Redox factor-1, AP endonuclease 1, APEX, APX, Protein REF-1, REF-1, REF1

Gene ID: 328

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus