The Mouse Monoclonal anti-GST antibody (Clone 3D4) (ABIN400782) specifically detects GST in WB, EIA and IHC (fro).
The antibody is reactive with Schistosoma japonicum samples.
Suitable for most immunological techniques requiring high titer binding and lot-to-lotconsistency. Recommended Dilutions: ELISA: 1/20,000-1/100,000Western Blot: 1/1,000-1/10,000 Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.47 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 as buffer and 0.01 % (w/v) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C or below forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.
Expiry Date
12 months
Target
GST
(Glutathione S Transferase (GST))
Alternative Name
Glutathione-S-Transferase (GST)
Background
GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.Synonyms: GST26-Tag, Glutathione S-transferase Tag