GST antibody

Catalog No. ABIN3020561

Product Details anti-GST Antibody

Target: GST (Glutathione S Transferase (GST))

Reactivity: Schistosoma japonicum

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GST antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Purification: Affinity purification

Immunogen: Recombinant protein of human GST-Tag

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:2000, IF: 1: 500, IP: 1:100

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C / -80 °C

References for anti-GST antibody (ABIN3020561)

Yang, Gu, Cheung, Yan, Tong, Jiang, Yang: "InsP3R-SEC5 interaction on phagosomes modulates innate immunity to Candida albicans by promoting cytosolic Ca2+ elevation and TBK1 activity." in: BMC biology, Vol. 16, Issue 1, pp. 46, (2018) (PubMed).

Zhao, Tao, Qi, Xu, Yin, Peng: "Protective effect of dioscin against doxorubicin-induced cardiotoxicity via adjusting microRNA-140-5p-mediated myocardial oxidative stress." in: Redox biology, Vol. 16, pp. 189-198, (2018) (PubMed).

Gou, Kang, Dai, Wang, Li, Zhao, Zhang, Hua, Lu, Zhu, Li, Chen, Wu, Li, Fu, Li, Shi, Liu: "Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis." in: Cell, Vol. 169, Issue 6, pp. 1090-1104.e13, (2017) (PubMed).

Zhang, Cai, Lin, Tao, Jin: "Subcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70." in: Virology journal, Vol. 14, Issue 1, pp. 30, (2017) (PubMed).

Zhang, Wang, Zhong, Luo, Shang, Liu, Chen, Fang, Xiao: "Ubiquitin-specific Protease 15 Negatively Regulates Virus-induced Type I Interferon Signaling via Catalytically-dependent and -independent Mechanisms." in: Scientific reports, Vol. 5, pp. 11220, (2016) (PubMed).

Target Details for GST

Target: GST (Glutathione S Transferase (GST))

Alternative Name: Glutathione-S-Transferase (GST) (GST Products)

Synonyms: GST antibody, GST 13-13 antibody, GST13 antibody, GST13-13 antibody, GSTK1-1 antibody, hGSTK1 antibody, 1500002K10Rik antibody, Gst antibody, Ptgds2 antibody, glutathione S-transferase kappa 1 antibody, microsomal glutathione S-transferase 1 antibody, hematopoietic prostaglandin D synthase antibody, GSTK1 antibody, Mgst1 antibody, Hpgds antibody

Background: Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. Forinstance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase(GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression bothin plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.