MAD2L1 antibody

Catalog No. ABIN400793

The Mouse Monoclonal anti-MAD2L1 antibody is suitable to detect MAD2L1 in samples from Human. It has been validated for WB and IP.

Quick Overview for MAD2L1 antibody (ABIN400793)

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAD2L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 17D10

Product Details anti-MAD2L1 Antibody

Specificity: This antibody is directed against MAD2L1 protein.

Characteristics: Synonyms: Mitotic spindle assembly checkpoint protein MAD2A, MAD2

Purification: Affinity chromatography on Protein A

Immunogen: Full-length recombinant human MAD2L1 protein

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1/200 - 1/2000. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MAD2L1

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Alternative Name: MAD2L1

Background: MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. This protein has a nuclear localization. A MAD2 pseudogene has been mapped to chromosome 14.Synonyms: MAD2, Mitotic spindle assembly checkpoint protein MAD2A

Gene ID: 4085

UniProt: Q13257