KLH antibody

Catalog No. ABIN400794

The Mouse Monoclonal anti-KLH antibody (Clone 12B4-G3-A8) (ABIN400794) specifically detects KLH in WB, IHC (fro) and EIA. The antibody is reactive with Keyhole Limpet samples.

Quick Overview for KLH antibody (ABIN400794)

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Reactivity: Keyhole Limpet

Host: Mouse

Clonality: Monoclonal

Conjugate: This KLH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 12B4-G3-A8

Product Details anti-KLH Antibody

Characteristics: Synonyms: Keyhole Limpet Hemocyaninlate

Purification: Affinity Chromatography on Protein A

Immunogen: A BALB/c mouse was immunized with KLH (Keyhole Limpet Hemocyanin)

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/20000 - 1/100000. Western Blot: 1/2000 - 1/10000. Immunohistochemistry: 1/1000 - 1/5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch

Expiry Date: 12 months

Target Details for KLH

Target: KLH (Keyhole Limpet Hemocyanin (KLH))

Background: KLH (Keyhole Limpet Hemocyanin) is a copper containing oxygen carrier occurring freely dissolved in the hemolymph of many molluscs and arthropods. KLH is often used in molecular immunology as a carrier protein conjugated to low molecular weight molecules such as peptides, amino acids, nucleic acids, drugs or toxins to render them more immunogenic due to the size of the conjugate complex and the immunogenicity of KLH. KLH forms a large complex composed of ~50 kDa subunits.Synonyms: Keyhole Limpet Hemocyanine