Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1) (AA 646-659) antibody

Antigen: Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1)

Synonyms: ADTG, CLAPG1, MGC18255, SYNG, AP1GBP1, FLJ34482, MGC104959, Adtg, AA409002, AU041323, AW551707, D8Ertd374e, adtg, zgc:56079, wu:fc30a11, AP1G1, DKFZp469F1514, ap1g1, MGC122095

Epitope: AA 646-659

Clonality: Polyclonal

Host: Goat

Reactivity: Chimpanzee

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN401169

Additional Information

Characteristics: Synonyms: AP1G1, ADTG, CLAPG1, gamma 1 Adaptin, gamma Adaptin, Adapter-related protein complex1 subunit gamma-1, Adaptor protein complex AP-1 subunit gamma-1, Golgi adaptorHA1/AP1, Clathrin assembly protein complex 1 gamma-1 large chain

Alternative name: AP1 complex subunit gamma-1

Swiss-Prot: O43747

Immunogen: Synthetic peptide corresponding aa 646-659 of Human AP1G1

Cross-Reactivity: Chimpanzee, Human

Isotype: IgG  (Matching secondary antibodies)

Description: AP1G1 (also known as gamma 1 subunit of adaptor -related protein complex 1,Gammaadaptin, Adaptor protein complex AP-1 gamma-1 subunit, Golgi adaptor HA1/AP1adaptin gamma-1 subunit and Clathrin assembly protein complex 1 gamma-1 large chain) isa subunit of clathrin-associated adaptor protein complex 1 that plays a role in proteinsorting in the late- Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexesmediate both the recruitment of clathrin to membranes and the recognition of sortingsignals within the cytosolic tails of transmembrane cargo molecules. The adaptin family ofproteins is composed of four classes of molecules named alpha, beta-, beta prime- andgamma- adaptins. Adaptins, together with medium and small subunits, form aheterotetrameric complex called an adaptor, whose role is to promote the formation ofclathrin-coated pits and vesicles. AP1G1 is localized to the coat surrounding thecytoplasmic face of coated vesicles located at the Golgi complex and is widely expressed.

Specificity: This antibody is directed against AP1G1. Species Reactivity: Tested: Human. Expected from sequence similarity: Chimpanzee, Orangutan, Mouse, Rat, Canine, Chicken.

Application Details

Application Notes: ELISA: 1/2,000 - 1/8,000. Western Blot: 1/500 - 1/2,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.7 mg/ml (by UV absorbance at 280 nm)

Purification: Aff - Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing0.01% (w/v) Sodium Azide

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only