RAD23B antibody (AA 163-176)

Catalog No. ABIN401177

The Goat Polyclonal anti-RAD23B antibody has been validated for WB and EIA. It is suitable to detect RAD23B in samples from Human, Dog, Chimpanzee and Saccharomyces cerevisiae.

Quick Overview for RAD23B antibody (AA 163-176) (ABIN401177)

Target: RAD23B (RAD23 Homolog B (RAD23B))

Reactivity: Human, Dog, Chimpanzee, Saccharomyces cerevisiae

Host: Goat

Clonality: Polyclonal

Conjugate: This RAD23B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RAD23B Antibody

Binding Specificity: AA 163-176

Specificity: Reactivity occurs against Human HR23B protein. Cross reactivity with HR23B protein from Mouse and Rat may also occur as sequence homology varies by one amino acid residue in this sequence by BLAST analysis. Reactivity with HR23B protein from other sources is not known.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Chimpanzee, S.cerevisiae.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase

Immunogen: Synthetic peptide corresponding aa 163-176 of human HR23B protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/2000 - 1/10000. Western Blot: 1/500 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for RAD23B

Target: RAD23B (RAD23 Homolog B (RAD23B))

Alternative Name: RAD23B

Background: HR23B (also known as UV excision repair protein RAD23 homolog B, XP-C repair complementing complex 58 kDa protein and p58) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.Synonyms: HR23B, UV excision repair protein RAD23 homolog B, XP-C repair-complementing complex 58 kDa protein

Gene ID: 5887

NCBI Accession: NP_002865

UniProt: P54727

Pathways: DNA Damage Repair