CBLC antibody (AA 444-458)

Catalog No. ABIN401316

Product Details anti-CBLC Antibody

Target: CBLC (Cbl proto-oncogene C (CBLC))

Binding Specificity: AA 444-458

Reactivity: Human, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBLC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Specificity: This antibody is directed against Cbl-c protein. No reactivity is expected with Cbl-a or Cbl-b.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human, Chimpanzee.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids 444-458 of Human Cbl-c

Isotype: IgG

Application Details

Application Notes: Immunoprecipitation. ELISA: 1/10,000 - 1/50,000. Western Blot: 1/500 - 1/3,000. Immunohistochemistry: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.42 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CBLC

Target: CBLC (Cbl proto-oncogene C (CBLC))

Alternative Name: CBLC / RNF57 (CBLC Products)

Synonyms: CBL-3 antibody, CBL-SL antibody, RNF57 antibody, Cbl proto-oncogene C antibody, CBLC antibody, Cblc antibody

Background: Cbl-c is also known as signal transduction protein CBL-C, SH3-binding protein CBL-C, CBL-3, and RING finger protein 57. Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cbl-c (a.k.a. Cbl-3) is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed). Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Cbl-c knock out mice show no obvious phenotype. Thus, the physiological role of Cbl-c is not known.Synonyms: CBL-3, CBL-C, CBL3, RING finger protein 57, SH3-binding protein CBL-C, Signal transduction protein CBL-C

Gene ID: 23624

NCBI Accession: NP_001124324

UniProt: Q9ULV8

Pathways: EGFR Signaling Pathway