Mre11 antibody (AA 68-706)

Catalog No. ABIN401319

The Rabbit Polyclonal anti-Mre11 antibody (ABIN401319) specifically detects Mre11 in WB and EIA. The antibody is reactive with Human, Rat, Mouse, Dog and Macaque samples.

Quick Overview for Mre11 antibody (AA 68-706) (ABIN401319)

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Reactivity: Human, Rat, Mouse, Dog, Macaque

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mre11 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Mre11 Antibody

Binding Specificity: AA 68-706

Specificity: This antibody is directed against Mre11 protein.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Macaque, Human, Canine.
Species reactivity (tested):Mouse.

Purification: Affinity Purified

Immunogen: Recombinant protein corres-ponding to amino acids 68-706 of mouse Mre11 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10000 - 1/50000. Western Blot: 1/500 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.05 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Mre11

Target: Mre11 (MRE11A) (MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A))

Alternative Name: MRE11

Background: Mre11 is a component of the MRN complex (Mre11/Rad50/Nbs1), which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses singlestrand endonuclease activity and doublestrand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. Mre11 is a nuclear protein that is widely expressed and localizes to discrete nuclear foci after treatment with genotoxic agents (including irradiation). Alternative splice isoforms are recorded for this protein.Synonyms: Double-strand break repair protein MRE11A, HNGS1, MRE11 homolog 1, MRE11 meiotic recombination 11 homolog A, MRE11A

Gene ID: 17535

NCBI Accession: NP_061206

UniProt: Q61216

Pathways: DNA Damage Repair