SMAD3 antibody (C-Term)

Catalog No. ABIN401333

The Rabbit Polyclonal anti-SMAD3 antibody is suitable to detect SMAD3 in samples from Human, Mouse, Rat, Pig, Chicken, Zebrafish (Danio rerio), Cow and Xenopus laevis. It has been validated for WB, IHC (p) and EIA.

Quick Overview for SMAD3 antibody (C-Term) (ABIN401333)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivity: Human, Mouse, Rat, Pig, Chicken, Zebrafish (Danio rerio), Cow, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SMAD3 Antibody

Binding Specificity: AA 417-425, C-Term

Specificity: This antibody is directed against Smad3 protein. ELISA and western blot show equivalent reactivity against phosphorylated and non-phosphorylated Human Smad3.

Cross-Reactivity (Details): Species reactivity (expected):Xenopus laevis, Xenopus tropicalis, Zebrafish, Rat, Mouse, Porcine, Bovine, Chicken.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Synthetic peptide corresponding to amino acids 417-425 of Human SMAD3 protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/10,000 - 1/40,000. Western Blot: 1/500 - 1/3,000. Immunohistochemistry: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.17 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Alternative Name: SMAD3

Background: Smad3 (also known as Mothers against decapentaplegic homolog 3 Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. These activators exert diverse effects on a wide array of cellular processes. The Smad proteins mediate much of the signaling responses induced by the TGF-beta superfamily. Briefly, activated type I receptor phosphorylates receptor-activated Smads (RSmads) at their c-terminal two extreme serines in the SSXS motif, e.g. Smad2 and Smad3 proteins in the TGF-β pathway, or Smad1, Smad5 or Smad8 in the BMP pathway. Then the phosphorylated R-Smad translocated into nucleus, where they regulate transcription of target genes. Based on microarray and animal model experiments, Smad3 accounts for at least 80 % of all TGF-beta-mediated response.Synonyms: JV15-2, MAD homolog 3, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, SMAD family member 3, SMAD-3

Gene ID: 4088

NCBI Accession: NP_001138574

UniProt: P84022

Pathways: Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy