Tamalin/GRASP antibody (N-Term)

Catalog No. ABIN401381

The Rabbit Polyclonal anti-Tamalin/GRASP antibody is suitable to detect Tamalin/GRASP in samples from Mouse and Rat. It has been validated for WB, IP and EIA.

Quick Overview for Tamalin/GRASP antibody (N-Term) (ABIN401381)

Target: Tamalin/GRASP (GRASP) (GRP1 (General Receptor For phosphoinositides 1)-Associated Scaffold Protein (GRASP))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tamalin/GRASP antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-Tamalin/GRASP Antibody

Binding Specificity: N-Term

Specificity: This antibody is directed against Tamalin protein.

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to amino acids near the amino terminus of mouse Tamalin protein

Isotype: IgG

Application Details

Application Notes: ELISA: 1/4,000 - 1/20,000. Western Blot: 1/500 - 1/3,000. Immunoprecipitation: 1 μg.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.03 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for Tamalin/GRASP

Target: Tamalin/GRASP (GRASP) (GRP1 (General Receptor For phosphoinositides 1)-Associated Scaffold Protein (GRASP))

Alternative Name: GRASP

Background: Tamalin, also named General receptor for phosphoinositides 1-associated scaffold protein (GRASP) is a PDZ (post-synaptic density protein/Drosophila disc large tumor suppressor/zo-1) domaincontaining protein that interacts with group 1 metabotropic glutamate receptors (mGluRs). The PDZ domaincontaining amino-terminal half of Tamalin binds directly to the class I PDZ-binding motif of group 1 mGluRs. The carboxyl-terminal half of Tamalin binds to cytohesins, which are guanine nucleotide exchange factors (GEFs) specific for the ADP-ribosylation factor (ARF) family of small GTP-binding proteins. Tamalin forms a protein complex with group 1 mGluRs at the post-synaptic site of specific neuronal cells and serves as a key scaffold protein that links a complex formation between mGluR1a and cytohesins. It is reported that Tamalin plays a key role in the association of group 1 mGluRs with the ARF-specific GEF proteins and contributes to intracellular trafficking and the macromolecular organization of group 1 mGluRs at synapses.Synonyms: GRP1-associated scaffold protein, General receptor for phosphoinositides 1-associated scaffold protein

Gene ID: 56149

NCBI Accession: NP_062391

UniProt: Q9JJA9