SIPA1 antibody (N-Term)

Catalog No. ABIN401404

Product Details anti-SIPA1 Antibody

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIPA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (expected):Human, Rat.
Species reactivity (tested):Mouse.

Purification: Affinity chromatography

Immunogen: Synthetic peptide corresponding to a region near the amino terminus of mouse Sipa1

Isotype: IgG

Application Details

Application Notes: ELISA: 1/20,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1.25 - 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.25 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for SIPA1

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Alternative Name: SIPA1 / SPA1 (SIPA1 Products)

Synonyms: Spa1 antibody, SPA1 antibody, signal-induced proliferation-associated 1 antibody, Signal peptidase I (leader peptidase I) antibody, signal-induced proliferation associated gene 1 antibody, SIPA1 antibody, sipA1 antibody, Sipa1 antibody

Background: Sipa1 (signal-induced proliferation associated gene 1) is a mitogen-induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. Sipa1 is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.Synonyms: GTPase-activating protein Spa-1, Signal-induced proliferation-associated protein 1, Sipa-1, Spa-1, p130 SPA-1

Gene ID: 10090

UniProt: P46062

Pathways: Response to Water Deprivation