THRA antibody (Isoform 1, N-Term)

Catalog No. ABIN401406

The Rabbit Polyclonal anti-THRA antibody (ABIN401406) specifically detects THRA in WB and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for THRA antibody (Isoform 1, N-Term) (ABIN401406)

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This THRA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-THRA Antibody

Binding Specificity: Isoform 1, N-Term

Specificity: This antibody reacts with Human THRA protein. A BLAST analysis was used to suggest cross-reactivity with THRA from Mouse, Human and Rat based on a 100 % homology with the immunizing sequence. Cross-reactivity with THRA from other sources has not been determined.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide corresponding to a region near the amino terminus of Human THRA isoform 1 protein . Genename: THRA

Isotype: IgG

Application Details

Application Notes: ELISA: 1/650,000. Western Blot: 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.89 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for THRA

Target: THRA (Thyroid Hormone Receptor, alpha (THRA))

Alternative Name: THRA / ERBA1

Background: Thyroid hormone receptor alpha is a nuclear hormone receptor with high affinity for the hormone triiodothyronine. THRA is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having a certain extent of redundancy, may mediate different functions of thyroid hormone. THRA interacts with NCOA3 and NCOA6 coactivators, leading to a strong increase in transcription of target genes. THRA is localized within the nucleus and has been found to exist as 4 isoforms originating from alternative splicing variants. This antibody recognizes THRA isoform 1. Isoform 1 has a distinct C-terminus compared to isoform 2.Synonyms: C-erbA-alpha, EAR-7, EAR7, NR1A1, Nuclear receptor subfamily 1 group A member 1, THRA1, THRA2, Thyroid hormone receptor alpha, c-erbA-1

Gene ID: 7067

NCBI Accession: NP_001177847

UniProt: P10827

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Sensory Perception of Sound, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Maintenance of Protein Location, Skeletal Muscle Fiber Development