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SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (Ser185)

Details for Product anti-SAE1 Antibody No. ABIN401468, Supplier: Login to see New
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(40), (8), (7), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Cow (Bovine), Dog (Canine), Chimpanzee, Human, Mouse (Murine), Rat (Rattus)
(128), (32), (27), (9), (8), (2), (2), (1), (1), (1)
(84), (27), (16), (1)
This SAE1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Enzyme Immunoassay (EIA), Western Blotting (WB)
(112), (66), (35), (27), (21), (10), (4), (4), (4), (1), (1), (1), (1)
Pubmed 3 references available
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Quantity 25 μL
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein
Isotype IgG
Specificity This antibody is directed against SUMO Activating Enzyme E1 protein.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Canine, Chimpanzee, Mouse, Rat.
Species reactivity (tested):Human.
Purification Affinity chromatography
Alternative Name SAE1 / AOS1 (SAE1 Antibody Abstract)
Background SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitinlike SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A
Gene ID 10055
NCBI Accession NP_005491
UniProt Q9UBE0
Application Notes ELISA: 1/100,000 - 1/125,000. Western Blot: 1/750 - 1/1,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.71 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
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 image for anti-SAE1 antibody (SUMO1 Activating Enzyme Subunit 1) (Ser185) (ABIN401468) anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (Ser185) antibody
Background publications Lois, Lima: "Structures of the SUMO E1 provide mechanistic insights into SUMO activation and E2 recruitment to E1." in: The EMBO journal, Vol. 24, Issue 3, pp. 439-51, 2005 (PubMed).

Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Catalog No. ABIN401468
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