CXCL2 antibody (C-Term)

Catalog No. ABIN401516

Product Details anti-CXCL2 Antibody

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CXCL2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: c-KKIIEKMLKN GKSN

Specificity: This antibody detects CXCL2 / MIP-2 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptid

Immunogen: Peptide from the C-Terminus of the protein sequence according to NP_002080.1. Genename: CXCL2

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.2-0.6 μg/mL. Approx 17Da band observed in Human Duodenum lysates(calculated MW of 11.4 kDa according to NP_002080.1).

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CXCL2

Target: CXCL2 (Chemokine (C-X-C Motif) Ligand 2 (CXCL2))

Alternative Name: CXCL2 / MIP2A (CXCL2 Products)

Synonyms: CINC-2a antibody, GRO2 antibody, GROb antibody, MGSA-b antibody, MIP-2a antibody, MIP2 antibody, MIP2A antibody, SCYB2 antibody, GRO3 antibody, Gro2 antibody, MIP-2 antibody, Mgsa-b antibody, Mip2 antibody, Scyb antibody, Scyb2 antibody, Mip-2 antibody, GROB antibody, MIP-2alpha antibody, C-X-C motif chemokine ligand 2 antibody, chemokine (C-X-C motif) ligand 2 antibody, CXCL2 antibody, Cxcl2 antibody

Background: The GRO gene was originally identified by subtractive hybridization studies between normal and tumorigenic Chinese hamster embryo fibroblasts. The hamster cDNA was cloned and used as a probe for cloning of the human GRO cDNA. The GROalpha gene initially cloned from T24 cells and the gene in melanoma cells encoding melanoma growth stimulating protein (MGSA) are identical. Human cells contain three closely related, but distinct GRO genes: GRO alpha, GRO beta, and GRO gamma. GRO beta and GRO gamma share 93 % and 82 % identity, respectively, with GRO alpha at the nucleotide level. GROs are members of the chemokine alpha family that is characterized by the separation with one amino acid of the first two cysteine residues, C-X-C, in the amino acid sequence. The GRO gene has been mapped to chromosome 4q21. In normal cells, human mRNA GRO expression is found in foreskin fibroblasts, synovial fibroblasts, chondrocytes and osteocytes. Additionally, GRO mRNA has been detected in mammary fibroblasts, mammary epithelial cells, endothelial cells, activated monocytes, macrophages, and neutrophils. Characterization of the GROalpha receptor indicates the presence of low and high affinity receptors on human neutrophils.Synonyms: C-X-C motif chemokine 2, GRO2, GROB, Gro-beta, Growth-regulated protein beta, MIP-2A, MIP2-alpha, Macrophage inflammatory protein 2-alpha, SCYB2

Gene ID: 2920

NCBI Accession: NP_002080

UniProt: P19875

Pathways: Cellular Response to Molecule of Bacterial Origin