HMOX2 antibody (Internal Region)

Catalog No. ABIN401519

Product Details anti-HMOX2 Antibody

Target: HMOX2 (Heme Oxygenase (Decycling) 2 (HMOX2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This HMOX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: c-DQAGSTLARE TLED

Specificity: This antibody detects HMOX2 (Internal).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_002125.3 . Genename: HMOX2

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: Recommended concentration: 0.3-1 μg/mL. Approx 37 kDa band observed inlysates of cell line K562 (calculated MW of 36.7 kDa according to NP_002125.3).

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for HMOX2

Target: HMOX2 (Heme Oxygenase (Decycling) 2 (HMOX2))

Alternative Name: Heme Oxygenase 2 (HMOX2) (HMOX2 Products)

Synonyms: HO-2 antibody, HO2 antibody, HMOX2 antibody, Ho-2 antibody, im:6909100 antibody, si:dkey-44g23.7 antibody, AtHO2 antibody, T9J22.22 antibody, T9J22_22 antibody, heme oxygenase 2 antibody, hmox2 antibody, ho-2 antibody, heme oxygenase 2 antibody, Heme oxygenase 2 antibody, heme oxygenase 2, putative antibody, heme oxygenase 2a antibody, heme oxygenase 2 L homeolog antibody, HMOX2 antibody, Hmox2 antibody, HO2 antibody, ho2 antibody, CNF01200 antibody, CPR_0203 antibody, CGB_F5120W antibody, hmox2a antibody, hmox2.L antibody

Background: Heme oxygenase (HO) is a microsomal enzyme involved in cellular response to oxidative stress. HO oxidatively cleaves the heme ring at the alpha methene bridge to form carbon monoxide and biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Two heme oxygenase isozymes, termed HO 1 and HO 2, have been identified so far. At the amino acid level, HO 1 and HO 2 are 42 % homologous. However, each isozyme is evolutionary highly conserved. HO 1 is the inducible form of the enzyme and is therefore also referred to as stress protein Hsp32. Expression of HO 1 can be induced by a variety of stimuli such as heme, metals, hormones, UV radiation and sulfhydryl depleting agents. In contrast, HO 2 is the constituitive form of the enzyme. It is not inducible by exogeneous stimuli and HO 2 is the most prevalent form in most tissues, except the spleen where HO 1 levels are predominant under normal conditions.Synonyms: HO-2, HO2

Gene ID: 3163

NCBI Accession: NP_002125

UniProt: P30519

Pathways: Transition Metal Ion Homeostasis