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GATA3 antibody (GATA Binding Protein 3) (N-Term)

Details for Product anti-GATA3 Antibody No. ABIN401530, Supplier: Log in to see
Antigen
  • GATA-3
  • XGATA3
  • hdr
  • xGATA-3
  • GATA3
  • gata3
  • HDR
  • HDRS
  • xgata-3
  • Gata-3
  • jal
  • cb550
  • gta3
Alternatives
anti-Human GATA3 antibody for Immunohistochemistry
Epitope
N-Term
23
20
20
16
15
13
12
8
7
6
5
4
3
3
3
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
195
99
41
10
10
8
6
6
5
2
1
1
1
Host
Goat
117
61
18
13
Clonality
Polyclonal
Conjugate
This GATA3 antibody is un-conjugated
7
5
5
5
5
5
5
4
3
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
145
82
56
27
25
22
19
16
6
5
2
1
1
1
Supplier
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Immunogen Peptide from the N Terminus of the protein sequence according to NP_001002295.1, NP_002042.1 . Genename: GATA3
Sequence EVTADQPRWV SHH-C
Specificity This antibody detects GATA3 at N-term. It is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Dog, Pig, Cow.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name GATA3 (GATA3 Antibody Abstract)
Background The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.Synonyms: GATA-3, GATA-binding factor 3, Trans-acting T-cell-specific transcription factor GATA-3
Gene ID 2625
NCBI Accession NP_001002295
UniProt P23771
Research Area Lineage Markers, Transcription Factors, Adaptive Immunity
Pathways TCR Signaling
Application Notes Peptide ELISA: Antibody detection limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 50 kDa band observed in lysates of cell line MOLT4(calculated MW of 48.0 according to NP_001002295.1 and 47.0 kDa according toNP_002042.1). Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells inthe interfollicular area and in germinal centres. Recommended concentration, 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Supplier Images
 image for anti-GATA3 antibody (GATA Binding Protein 3) (N-Term) (ABIN401530) anti-GATA Binding Protein 3 (GATA3) (N-Term) antibody
 image for anti-GATA3 antibody (GATA Binding Protein 3) (N-Term) (ABIN401530) anti-GATA Binding Protein 3 (GATA3) (N-Term) antibody (Image 2)
Background publications Van Opstal, Hepp, Suzuki et al.: "Role of monkey nucleus reticularis tegmenti pontis in the stabilization of Listing's plane." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 16, Issue 22, pp. 7284-96, 1996 (PubMed).

Marine, Winoto: "The human enhancer-binding protein Gata3 binds to several T-cell receptor regulatory elements." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 16, pp. 7284-8, 1991 (PubMed).