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Details for Product No. ABIN401600

Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pThr75) antibody

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Antigen
Synonyms Darpp32, AU040756, DARPP-32, DARPP32, Darpp-32, darpp32, darpp-32
Epitope
»Alternatives pThr75
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunocytochemistry (ICC), Immunofluorescence (IF)
Pubmed 3 references available
Catalog no. ABIN401600
Quantity 0.1 mg
Price
346.50 $   Plus shipping costs $45.00
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Immunogen Synthesized phosphopeptide derived from human DARPP-32 around the phosphorylation site of threonine 75 (A-Y-TP-P-P).
Specificity DARPP-32 (Phospho-Thr75) Antibody detects endogenous levels of DARPP-32 only when phosphorylated at threonine 75.
Purification Affinity chromatography
Alternative Name DARPP32
Background DARPP32 is expressed in medium-sized spiny neurons that also express dopamine D1 receptors. The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32 and phosphorylated DARPP32 is a potent protein phosphatase 1. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho DARPP32, thereby reducing the phosphatase 1 inhibitory activity of DARPP32.
Alternate names: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B
Gene ID 84152
NCBI Accession NP_115568
UniProt Q9UD71
Research Area Signaling
Application Notes Immunofluorescence: 1/100 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Background publications Nishi, Snyder, Greengard: "Bidirectional regulation of DARPP-32 phosphorylation by dopamine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 21, pp. 8147-55, 1997 (PubMed).

Bibb, Snyder, Nishi et al.: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, 2000 (PubMed).

Alves, Régulier, Nascimento-Ferreira et al.: "Striatal and nigral pathology in a lentiviral rat model of Machado-Joseph disease." in: Human molecular genetics, Vol. 17, Issue 14, pp. 2071-83, 2008 (PubMed).

Alternatives for antigen "Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B)", type "Antibodies"
Hosts (125), (6), (2), (2)
Reactivities (114), (101), (88), (51), (49), (36), (36), (12), (4), (4), (2), (1), (1)
Applications (98), (45), (35), (30), (22), (20), (7), (6), (3), (2), (1), (1), (1)
Conjugates (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (36), (28), (11), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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