Calnexin antibody (Internal Region)

Catalog No. ABIN401659

This Goat Polyclonal antibody specifically detects Calnexin in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Dog, Cow and Pig. It has been mentioned in 2+ publications

Quick Overview for Calnexin antibody (Internal Region) (ABIN401659)

Target: Calnexin (CANX)

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Calnexin Antibody

Binding Specificity: Internal Region

Sequence: c-SKTPELNLDQ FHDKT

Specificity: This antibody recognizes Calnexin.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine, Pig, Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region (near N-Terminus) of the protein sequence . Genename: CANX

Application Details

Application Notes: Peptide ELISA: 1/32000. Western blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Calnexin antibody (ABIN401659)

Ho, Chang, Su, Chang, Hu, Jou, Wang: "Inhibition of matrix metalloproteinase activity reverses corneal endothelial-mesenchymal transition." in: The American journal of pathology, Vol. 185, Issue 8, pp. 2158-67, (2016) (PubMed).

Cordek, Croom-Perez, Hwang, Hargittai, Subba-Reddy, Han, Lodeiro, Ning, McCrory, Arnold, Koc, Lindenbach, Showalter, Cameron: "Expanding the proteome of an RNA virus by phosphorylation of an intrinsically disordered viral protein." in: The Journal of biological chemistry, Vol. 289, Issue 35, pp. 24397-416, (2015) (PubMed).

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin

Background: Calnexin, also referred to as IP90, p88 and p90, is an ~90 kDa integral membrane protein of the endoplasmic reticulum (ER). Many resident ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multisubunit proteins. Studies indicate that calnexin associates with the major histocompatability complex (MHC) class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin, but not with completed receptor complexes. It has been shown that calnexin is a chaperone that retains incompletely or improperly folded proteins in the ER. The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble ER resident proteins such as GRP 78 and GRP 94 and protein disulfide isomerase. Integral membrane ER resident proteins, like calnexin, often lack this KDEL sequence but contain positively charged cytosolic residues that ensure ER retention. Calnexin contains a large ER luminal domain (461 amino acids), a transmembrane segment (22 amino acids), and a cytoplasmic tail (89 amino acids). These features distinguish calnexin from soluble ER chaperones that cannot interact with the transmembrane and cytosolic domains of integral membrane proteins. The amino acid sequence of calnexin is highly conserved among species, and shares regions of high sequence homology with calreticulin.Synonyms: CANX, ER Marker, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90

Gene ID: 821

NCBI Accession: NP_001737

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis