P2RX7 antibody (N-Term)

Catalog No. ABIN401660

There is 1 publication for this product available. The Goat Polyclonal anti-P2RX7 antibody is suitable to detect P2RX7 in samples from Human, Mouse, Rat and Dog. It has been validated for WB, IHC (p) and EIA.

Quick Overview for P2RX7 antibody (N-Term) (ABIN401660)

Target: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This P2RX7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-P2RX7 Antibody

Binding Specificity: N-Term

Sequence: YETNKVTRIQ SMNY-C

Specificity: This antibody recognizes P2X7 receptor.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Peptide with sequence from the N-Terminus of the protein sequence (NP_002553.2). Genename: P2RX7

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-P2RX7 antibody (ABIN401660)

Graziano, Desdouits, Garzetti, Podini, Alfano, Rubartelli, Furlan, Benaroch, Poli: "Extracellular ATP induces the rapid release of HIV-1 from virus containing compartments of human macrophages." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 25, pp. E3265-73, (2015) (PubMed).

Target Details for P2RX7

Target: P2RX7 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))

Alternative Name: P2RX7 / P2X7

Background: The product P2RX7 belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression.Synonyms: ATP receptor, P2X purinoceptor 7, P2Z receptor, Purinergic receptor

Molecular Weight: 68.6kDa (NP_002553.2).

Gene ID: 5027

NCBI Accession: NP_002553

UniProt: Q99572

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Vesicle Exocytosis