Nuclear Transcription Factor, X-Box Binding 1 (NFX1) (N-Term) antibody

Details for Product No. ABIN404964
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Antigen
Synonyms NFX1, NFX2, 1300017N15Rik, 3000003M19Rik, NFX.1, TEG-42, Tex42
Epitope
N-Term
(7), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine), Human
(31), (15), (15), (1), (1)
Host
Rabbit
(21), (10)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(21), (11), (10), (6), (4), (3), (3), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN404964
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human NFX1
Sequence MAEAPPVSGT FKFNTDAAEF IPQEKKNSGL NCGTQRRLDS NRIGRRNYSS
Predicted Reactivity Human : 100 %, Bovine : 90 %, Dog : 90 %, Horse : 90 %, Mouse : 90 %, Pig : 90 %, Rabbit : 90 %, Rat : 90 %
Characteristics This is a rabbit polyclonal antibody against NFX1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name NFX1
Background NFX1 binds to the X-box motif of MHC class II genes and represses their expression. NFX1 may play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression. NFX1 mediates E2-dependent ubiquitination.MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.
Molecular Weight 113 kDa
Gene ID 4799
NCBI Accession NM_147133, NP_667344
Research Area Chromatin and Nuclear Signaling, Transcription Factors, Proteolysis / Ubiquitin, Adaptive Immunity
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 1024 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Nuclear Transcription Factor, X-Box Binding 1 (NFX1) (N-Term) antibody WB Suggested Anti-NFX1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate
Background publications Katzenellenbogen, Egelkrout, Vliet-Gregg et al.: "NFX1-123 and poly(A) binding proteins synergistically augment activation of telomerase in human papillomavirus type 16 E6-expressing cells." in: Journal of virology, Vol. 81, Issue 8, pp. 3786-96, 2007 (PubMed).

Validation Images
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