HSD3B2 antibody (N-Term)

Catalog No. ABIN405635

The Rabbit Polyclonal anti-HSD3B2 antibody is suitable to detect HSD3B2 in samples from Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Sheep and Monkey. It has been validated for WB and IHC.

Quick Overview for HSD3B2 antibody (N-Term) (ABIN405635)

Target: HSD3B2 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 2 (HSD3B2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Sheep, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD3B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-HSD3B2 Antibody

Binding Specificity: N-Term

Purpose: HSD3B2 Antibody - N-terminal region

Sequence: GWSCLVTGAG GLLGQRIVRL LVEEKELKEI RALDKAFRPE LREEFSKLQN

Predicted Reactivity: Cow: 85%, Dog: 93%, Goat: 85%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 79%, Rat: 86%, Sheep: 85%

Characteristics: This is a rabbit polyclonal antibody against HSD3B2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HSD3B2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP44239-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for HSD3B2

Target: HSD3B2 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 2 (HSD3B2))

Alternative Name: HSD3B2

Background:

Background Information: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.

Gene Name: Hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2

Alternative Symbols: HSDB, HSD3B, SDR11E2

Protein Name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 2

Molecular Weight: 42kDa

Gene ID: 3284

NCBI Accession: NP_000189

UniProt: P26439

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis