POLR2K antibody (Middle Region)

Catalog No. ABIN406007

The Rabbit Polyclonal anti-POLR2K antibody is suitable to detect POLR2K in samples from Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog and Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for POLR2K antibody (Middle Region) (ABIN406007)

Target: POLR2K (Polymerase (RNA) II (DNA Directed) Polypeptide K, 7.0kDa (POLR2K))

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2K antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR2K Antibody

Binding Specificity: Middle Region

Purpose: POLR2K Antibody - middle region

Sequence: DTQKDVQPPK QQPMIYICGE CHTENEIKSR DPIRCRECGY RIMYKKRTKR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against POLR2K. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLR2K

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48658-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR2K

Target: POLR2K (Polymerase (RNA) II (DNA Directed) Polypeptide K, 7.0kDa (POLR2K))

Alternative Name: POLR2K

Background:

Targeted Subunit: RPABC4

Background Information: POLR2K is one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases.This gene encodes one of the smallest subunits of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Gene Name: Polymerase (RNA) II (DNA directed) polypeptide K, 7.0 kDa

Alternative Symbols: RPB12, RPABC4, RPB7.0, hRPB7.0, hsRPB10a, RPB10alpha, ABC10-alpha

Protein Name: DNA-directed RNA polymerases I, II, and III subunit RPABC4

Molecular Weight: 7kDa

Gene ID: 5440

NCBI Accession: NP_005025

UniProt: P53803

Pathways: Regulatory RNA Pathways