ARHGAP25 antibody (Middle Region)

Catalog No. ABIN406687

This Rabbit Polyclonal antibody specifically detects ARHGAP25 in WB. It exhibits reactivity toward Human, Rat, Dog, Pig, Cow, Horse and Rabbit.

Quick Overview for ARHGAP25 antibody (Middle Region) (ABIN406687)

Target: ARHGAP25 (rho GTPase Activating Protein 25 (ARHGAP25))

Reactivity: Human, Rat, Dog, Pig, Cow, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGAP25 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ARHGAP25 Antibody

Binding Specificity: Middle Region

Purpose: ARHGAP25 Antibody - middle region

Sequence: SPGEEASALS SQACDSKGDT LASPNSETGP GKKNSGEEEI DSLQRMVQEL

Predicted Reactivity: Cow: 93%, Dog: 92%, Horse: 92%, Human: 100%, Pig: 86%, Rabbit: 92%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against ARHGAP25. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ARHGAP25

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP58423-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for ARHGAP25

Target: ARHGAP25 (rho GTPase Activating Protein 25 (ARHGAP25))

Alternative Name: ARHGAP25

Background:

Background Information: ARHGAPs, such as ARHGAP25, are negative regulators of Rho GTPases, which are implicated in actin remodeling, cell polarity, and cell migration.ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA, MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM].

Gene Name: Rho GTPase activating protein 25

Alternative Symbols: KAIA0053, HEL-S-308

Protein Name: Rho GTPase-activating protein 25

Molecular Weight: 70kDa

Gene ID: 9938

NCBI Accession: NP_055697

UniProt: P42331