Tissue Specific Transplantation Antigen P35B (TSTA3) (N-Term) antibody

Details for Product No. ABIN406731
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Antigen
Synonyms p35b, sdr4e1, wu:fe16c04, TSTA3, zgc:101805, si:dkey-235d18none, FX, P35B, SDR4E1, AI256181, Tstap35b, Fx
Epitope
N-Term
(11), (8), (7), (3), (2), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine), Human
(41), (22), (14)
Host
Rabbit
(36), (13)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(49), (37), (8), (3), (2), (1)
Pubmed 1 reference available
Quantity 50 µg
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Catalog No. ABIN406731
289.00 $
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Immunogen Synthetic peptide directed towards the N terminal of human TSTA3
Sequence MGEPQGSMRI LVTGGSGLVG KAIQKVVADG AGLPGEDWVF VSSKDADLTD
Predicted Reactivity Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rabbit : 100 %, Rat : 100 %, Horse : 90 %, Bovine : 80 %
Characteristics This is a rabbit polyclonal antibody against TSTA3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name TSTA3
Background Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II.Tissue specific transplantation antigen P35B is a NADP(H)-binding protein. It catalyze the two-step epimerase and the reductase reactions in GDP-D-mannose metabolism, converting GDP-4-keto-6-D-deoxymannose to GDP-L-fucose. GDP-L-fucose is the substrate of several fucosyltransferases involved in the expression of many glycoconjugates, including blood group ABH antigens and developmental adhesion antigens. Mutations in this gene may cause leukocyte adhesion deficiency, type II.
Molecular Weight 35 kDa
Gene ID 7264
NCBI Accession NP_003304, NM_003313
UniProt Q13630
Research Area Signaling, Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 321 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Tissue Specific Transplantation Antigen P35B (TSTA3) (N-Term) antibody WB Suggested Anti-TSTA3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: MCF7 cell lysate
anti-Tissue Specific Transplantation Antigen P35B (TSTA3) (N-Term) antibody (2) Host: Rabbit
Target Name: TSTA3
Sample Tissue: 721_B
Antibody Dilution: 1.0 µg/mL TSTA3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
anti-Tissue Specific Transplantation Antigen P35B (TSTA3) (N-Term) antibody (3) Host: Rabbit
Target Name: TSTA3
Sample Tissue: Hela
Antibody Dilution: 1.0 µg/mL TSTA3 is strongly supported by BioGPS gene expression data to be expressed in HeLa
anti-Tissue Specific Transplantation Antigen P35B (TSTA3) (N-Term) antibody (4) Rabbit Anti-TSTA3 Antibody ,Paraffin Embedded Tissue: Human Adult Liver
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:000
Magnification: 00X
Exposure Time: 0.5 - 2.0 sec
Protocol located in Reviews and Data.
Background publications Roos, Kolmer, Mattila et al.: "Composition of Drosophila melanogaster proteome involved in fucosylated glycan metabolism." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3168-75, 2002 (PubMed).

Validation Images
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