F2R
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
6A7H10
unconjugated
Application Notes
Peptide ELISA: Detection limit dilution: 1/32000. Western blot: 0.5-2 μg/mL. Approx 48 kDa band observed in lysates of cell line HeLa(calculated MW of 47.8 kDa according to NP_001983.2). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 with 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Target
PAR1 (F2R)
(Coagulation Factor II (thrombin) Receptor (F2R))
Thrombin receptor is a 7 transmembrane G protein coupled receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. It may play a role in platelet activation and in vascular development. Thrombin receptor expression has been documented in various blood cells, colon, kidney, liver, lung, skin, and vessel. ESTs have been isolated from adrenal, embryo, heart, heart/melanocyte/uterus, liver/spleen, pineal, skin, spleen, and vessel.Synonyms: CF2R, Coagulation factor II receptor, PAR-1, PAR1, Proteinase-activated receptor 1