Smooth Muscle Actin antibody (N-Term)

Catalog No. ABIN452371

This Mouse Monoclonal antibody specifically detects Smooth Muscle Actin in WB, IF, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Rat, Mouse, Chicken, Cow and Horse.

Quick Overview for Smooth Muscle Actin antibody (N-Term) (ABIN452371)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Reactivity: Human, Rat, Mouse, Chicken, Cow, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Smooth Muscle Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ASM-1

Product Details anti-Smooth Muscle Actin Antibody

Binding Specificity: N-Term

Specificity: ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. Polypeptide reacting: specific for alpha-smooth-muscle isoform of actin (Mr 43,000). Tumors Specificallly Detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast. Tested Reactivities on Cultured Cell lines: Stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Equine, Rat, Mouse and Chicken.

Purification: Affinity Chromatography on Protein A

Immunogen: Synthetic N- terminus decapeptide of alpha-smooth-muscleisoform of Actin.

Isotype: IgG2a

Application Details

Application Notes: Immunofluorescence MicroscopyImmunoblotting (Western). Cytological Material. Immunohistochemistry on Frozen and Paraffin-Embedded Tissue. Pretreatment: With paraffin embedded sections, protease pretreatment is required prior toantibody application. Working Dilution: Ready-to-use for Immunohistochemical application. Incubation Time: 1 h at RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluited at 2-8 °CShelf life: One year from despatch.

Expiry Date: 12 months

Target Details for Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Background: The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.Synonyms: ACTSA, ACTVS, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

Gene ID: 58

UniProt: P68133

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development