JunB antibody (Internal Region)

Catalog No. ABIN452429

The Goat Polyclonal anti-JunB antibody is suitable to detect JunB in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB.

Quick Overview for JunB antibody (Internal Region) (ABIN452429)

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This JunB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-JunB Antibody

Binding Specificity: Internal Region

Sequence: c-TLKAENAGLS STAG

Specificity: This antibody recognizes JUNB.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Rat, Mouse and Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_002220.1.

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: Use at 0.3-1.0 μg/mL. Approx 35 kDa band observed in Human Breast cancer lysates(Calculated MW of 35.5 kDa according to NP_002220.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris-saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for JunB

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Alternative Name: Jun-B

Background: The cJun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus vjun oncogene. JunB and JunD have been shown to be almost identical to cJun in their C terminal regions, which are involved in dimerization and DNA binding, whereas their N terminal domains, which are involved in transcriptional activation, diverge. JunB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3'.Synonyms: JUNB, Transcription factor jun-B

Gene ID: 3726

NCBI Accession: NP_002220

UniProt: P17275