Human Immunodeficiency Virus Type 1 (HIV-1) antibody
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- Target
- Human Immunodeficiency Virus Type 1 (HIV-1)
- Reactivity
- Human Immunodeficiency Virus (HIV)
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Cross-Reactivity (Details)
- Species reactivity (tested):Human.
- Characteristics
- Synonyms: Human immunodeficiency virus type 1, HIV-1 P24, Capsid protein p24
- Purification
- Affinity Chromatography on Protein G.
- Immunogen
- Purified Recombinant HIV-1 P24 (Core Protein).
- Clone
- 1027
- Isotype
- IgG1
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- Application Notes
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ELISA. Western Blot: 0.1-0.5 μg/mL of HIV1 antibody (clone 1027) is used with 0.1 μg/lane ofantigen in reduced SDS-PAGE gel then blotted on PVDF membrane.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.
- Buffer
- 0.01 M PBS, pH 7.2 without preservatives.
- Preservative
- Without preservative
- Storage
- -20 °C
- Storage Comment
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Store the antibody at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- Human Immunodeficiency Virus Type 1 (HIV-1)
- Alternative Name
- HIV-1
- Target Type
- Virus
- Background
- HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.Synonyms: Capsid protein p24, HIV-1 P24, Human immunodeficiency virus type 1
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