TFB1M antibody (C-Term)

Catalog No. ABIN452674

This Goat Polyclonal antibody specifically detects TFB1M in WB and EIA. It exhibits reactivity toward Human and Dog.

Quick Overview for TFB1M antibody (C-Term) (ABIN452674)

Target: TFB1M (Transcription Factor B1, Mitochondrial (TFB1M))

Reactivity: Human, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This TFB1M antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-TFB1M Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-ELKRRKSKNEEKE, from the C-Terminus of Human TFB1M (NP_057104.2).

Application Details

Application Notes: TFB1M antibody can be used for: Peptide ELISA: Detection limit dilution 1/64000. Western blot: 0.3-1 μg/mL. Approx 38 kDa band observed in lysates of cell line A431(calculated MW of 39.8 kDa according to NP_057104.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human. Expected from sequence similarity: Canine (Dog).

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for TFB1M

Target: TFB1M (Transcription Factor B1, Mitochondrial (TFB1M))

Alternative Name: TFB1M

Background: TFB1M is a S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. It is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. It stimulates transcription independently of the methyltransferase activity.Synonyms: CGI-75, Dimethyladenosine transferase 1 mitochondrial, Mitochondrial 12S rRNA dimethylase 1, Mitochondrial transcription factor B1, N'-adenosyl(rRNA) dimethyltransferase 1, S-adenosylmethionine-6-N', mtTFB1

Gene ID: 51106

NCBI Accession: NP_057104

UniProt: Q8WVM0