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PEA15 antibody (phosphoprotein Enriched in Astrocytes 15) (C-Term)

Details for Product anti-PEA15 Antibody No. ABIN453779, Supplier: Log in to see
Antigen
  • DKFZp459O1410
  • HMAT1
  • HUMMAT1H
  • MAT1
  • Mat1
  • MAT1H
  • PEA-15
  • Pea15
  • PEA15
  • Pea15a
  • PED
  • Pkcs15
Epitope
C-Term
29
15
10
5
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
104
64
54
7
1
1
Host
Rabbit
101
3
1
Clonality
Polyclonal
Conjugate
This PEA15 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
71
57
42
19
15
13
4
3
2
2
Supplier
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Immunogen KLH conjugated synthetic peptide selected from the C-terminal region of human PEA-15
Specificity This antibody reacts to PEA15.
Purification Affinity chromatography on Protein A
Alternative Name PEA15 (PEA15 Antibody Abstract)
Background PEA15 is a death effector domain (DED)-containing protein predominantly expressed in the central nervous system, particularly in astrocytes.Synonyms: Astrocytic phosphoprotein PEA-15, PED, Phosphoprotein enriched in diabetes
Gene ID 8682
NCBI Accession NP_003759
UniProt Q15121
Research Area Apoptosis/Necrosis
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-PEA15 antibody (phosphoprotein Enriched in Astrocytes 15) (C-Term) (ABIN453779) (LEFT) Western blot analysis of PEA-15 Antibody (C-term) in T47D cell line lysates (3...
Background publications Sugiyama, Masuda, Shinoda, Nakamura, Tomita, Ishihama: "Phosphopeptide enrichment by aliphatic hydroxy acid-modified metal oxide chromatography for nano-LC-MS/MS in proteomics applications." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 6, pp. 1103-9, 2007 (PubMed).

Trencia, Perfetti, Cassese, Vigliotta, Miele, Oriente, Santopietro, Giacco, Condorelli, Formisano, Beguinot: "Protein kinase B/Akt binds and phosphorylates PED/PEA-15, stabilizing its antiapoptotic action." in: Molecular and cellular biology, Vol. 23, Issue 13, pp. 4511-21, 2003 (PubMed).