Crystallin, alpha A (CRYAA) antibody

Antigen: Crystallin, alpha A (CRYAA)

Synonyms: CRYA1, HSPB4, Crya1, lop18, Acry-1, Crya-1, DAcry-1, AI323437, cryaa-A, CRYAA, fc06h04, MGC92036, zgc:92036, wu:fc06h04, alpha-a-crystallin

Clonality: Monoclonal (1H3-B8)

Host: Mouse

Reactivity: Cow (Bovine)

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN453938

Additional Information

Characteristics: Synonyms: CRYAA, CRYA1, HSPB4, Heat shock protein beta-4

Alternative name: Alpha-crystallin A chain

Gene ID: 1409

Swiss-Prot: P02489

Immunogen: Native alpha crystallin

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG1  (Matching secondary antibodies)

Clone: 1H3-B8

Description: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eyethat are related to the small heat shock protein family. The alphacrystallins possessstructural and functional similarities with Hsp25 and Hsp27 (1). Mammalian lens cystallinsare divided into alpha, beta and gamma families. Alpha and beta families are furtherdivided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens,alpha-crystallin primarily functions to maintain proper refractive index, however it can alsofunction as a molecular chaperone that binds to the denatured proteins, keeping them insolution and thereby maintaining the translucency of the lens. When cellular stress occurs,alpha-crystallin enters its' phosphorylated state and may serve a structural controlfunction and play a role in protein maintenance (2). In addition to their interaction withproteins, alpha-crystallins also interact with native molecules such as membrane proteins,Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Twoother functions are an autokinase activity and participation in the intracellulararchitecture, and it has also been proven that both alpha-A and B prevent apoptosis byinhibiting caspases (8).

Specificity: This antibody detects alpha crystallin at ~20kDa. Detects alpha crystallin complex (containing alpha A and alpha B subunits). Does not

Application Details

Application Notes: Western blot: 1/2000. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % azid

Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing. Shelf life: one year from despatch.

Research Area: Neurology, Enzymes

Restrictions: For Research Use only