CD4 Molecule (CD4) antibody (PE)

Antigen: CD4 Molecule (CD4)

Synonyms: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Clonality: Monoclonal (GK1-5)

Host: Rat

Reactivity: Mouse (Murine)

Conjugate: PE

Application: Flow Cytometry (FACS)

Catalog no.: ABIN457367

Additional Information

Alternative name: CD4

Immunogen: Mouse CTL clone V4 cells

Isotype: IgG2b

Clone: GK1-5

Description: CD4 is a single chain transmembrane glycoprotein of immunoglobulin supergene family. Inits extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3Ig-like C2-type). The intracellular region of CD4 associates with p56Lck, a Src-like proteintyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cellmembrane microdomains. CD4 binds to MHC class II molecules (by CDR2-like region inCD4 domain 1), HIV envelope protein gp120 (by CDR2-like region in CD4 domain 1) andother ligands, such as IL-16 (by to CD4 domain 3) or L-selectin. CD4 is a co-receptorinvolved in immune response (co-receptor activity in binding to MHC class II molecules) andHIV infection. CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cellselection and signal transduction. Defects in antigen presentation (MHC class II) causedysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue andorgans (SCID immunodeficiency).

Specificity: The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4transmembrane glycoprotein (55 kDa).

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 3 µg/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Concentration: 0.5 mg/ml

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only