Selectin P Ligand (SELPLG) antibody

Antigen: Selectin P Ligand (SELPLG)

Synonyms: CLA, CD162, PSGL1, PSGL-1, SELPLG, SELPG

Clonality: Monoclonal (TC2)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN457437

Additional Information

Alternative name: CD162

Format: Purified

Isotype: IgG1

Clone: TC2

Description: CD162 (P-selectin glycoprotein ligand-1, PSGL-1) is a sialomucin constitutively expressedas a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulatingleukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity forP-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite forfirm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediatesleukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhessionmolecule on mature leukocytes, CD162 is a potent negative regulator of humanhematopoietic progenitors.

Specificity: The antibody TC2 reacts with CD162, a 220 kDa type I integral membrane proteinexpressed as disulfide-linked homodimer (sialomucin family). CD162 is present on the mostperipheral blood T lymphocytes, monocytes, granulocytes, it is also expressed on asubpopulation of B lymphocytes and CD34 + bone marrow cells.

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 1. 5 - 2. 5 µg/ml. Positive control: human peripheral blood

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Cardiovascular, Atherosclerosis, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only