Mouse anti-Human IgA2 Antibody

Catalog No. ABIN457923

Quick Overview for Mouse anti-Human IgA2 Antibody (ABIN457923)

Target: IgA2

Clonality: Monoclonal

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Haemagglutination (H)

Clone: NI 512 (A89-038)

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the IgA2 molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Monoclonal mouse antiserum to human IgA2

Characteristics: Monoclonal mouse antiserum to human IgA2

Purification: Delipidated, heat inactivated, lyophilized, stable whole ascites

Immunogen: Highly purified monoclonal IgA2 isolated from pooled human serum

Application Details

Application Notes: To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgA2, haemagglutination and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.

Concentration: 1 mg/mL

Buffer: No foreign proteins added.

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgA2