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Mouse anti-Human IgA2 (Allotype IgA2(m)2) Antibody

ELISA, IF Monoclonal NI 194-3 (A89-040) IgG1 unconjugated
Catalog No. ABIN457924
  • Target See all IgA2 products
    IgA2
    Binding Specificity
    • 4
    • 3
    • 1
    • 1
    Allotype IgA2(m)2
    Reactivity
    Human
    Host
    • 11
    • 2
    Mouse
    Clonality
    • 11
    • 2
    Monoclonal
    Application
    • 10
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    ELISA, Immunofluorescence (IF)
    Specificity
    Monoclonal Mouse antiSerum to Human IgA2(m)2, allotype specific
    Cross-Reactivity (Details)
    The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
    Characteristics
    The reactivity of the antiserum is restricted to the IgA2(m)2 Molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.
    Purification
    Delipidated, heat inactivated, stable ascites
    Sterility
    Heat-inactivated
    Immunogen
    Highly purified monoclonal IgA2(m)2 isolated from human serum.
    Clone
    NI 194-3 (A89-040)
    Isotype
    IgG1
  • Application Notes
    Indirect immunofluorescence,ELISA.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/mL. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.
    Buffer
    Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/mL. No foreign proteins added.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.
  • Target
    IgA2
    Alternative Name
    IgA22 (IgA2 Products)
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